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Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test.

Salimi-Bejestani, M.R.; McGarry, J.W.; Felstead, S.; Ortiz-Oblitas, P.; Akça, A.; Williams, D.J.L. (Elsevier Ltd., 2005)

An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected ...

The specificity of antibody responses in cattle naturally exposed to Fasciola hepatica.

Ortiz-Oblitas, P.; Claxton, J.R.; Clarkson, M.J.; McGarry, J.W.; Williams, D.J.L. (Elsevier Ltd., 2000)

Fasciola hepatica causes significant morbidity and mortality in dairy cattle in the Andean region of Cajamarca, Peru, where prevalence of infection of up to 78% has been reported. ELISA and Western blot analyses were used ...

The egg hatch test: A useful tool for albendazole resistance diagnosis in Fasciola hepatica.

Ceballos, L.; Canton, C.; Pruzzo, C.; Sanabria, R.; Moreno, L.; Sanchís, J.; Suárez, G.; Ortiz-Oblitas, P.; Fairweather, I.; Lanusse, C.; Álvarez, L.; Martínez-Valladares, M.M. (Elsevier B.V., 2019)

In the current study, the egg hatch test (EHT) has been evaluated as an in vitro technique to detect albendazole (ABZ) resistance in Fasciola hepatica. The intra- and inter-assay variations of the EHT were measured by means ...

Testing albendazole resistance in Fasciola hepatica: Validation of an egg hatch test with isolates from South America and the United Kingdom.

Canevari, J.; Ceballos, L.; Sanabria, R.; Romero, J.; Olaechea, F.; Ortiz-Oblitas, P.; Cabrera, M.; Gayo, V.; Fairweather, I.; Lanusse, C.; Álvarez, L. (Cambridge University Press., 2014)

The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n= ...

A single amino acid substitution in isozyme GST mu in Triclabendazole resistant Fasciola hepatica (Sligo strain) can substantially influence the manifestation of anthelmintic resistance.

Fernández, V.; Estein, S.; Ortiz-Oblitas, P.; Luchessi, P.; Solana, V.; Solana, H. (Academic Press Inc., 2015)

The helminth parasite Fasciola hepatica causes fascioliasis in human and domestic ruminants. Economic losses due to this infection are estimated in U$S 2000-3000 million yearly. The most common method of control is the use ...

Molecular characterization and phylogenetic analysis of Fasciola hepatica from Peru.

Ichikawa-Seki, M.; Ortiz-Oblitas, P.; Cabrera, M.; Hobán-Vergara, C.; Itagaki, T. (Elsevier Ireland Ltd., 2016)

The causative agent of fasciolosis in South America is thought to be Fasciola hepatica. In this study, Fasciola flukes from Peru were analyzed to investigate their genetic structure and phylogenetic relationships with those ...

Fasciola hepatica phenotypic characterization in Andean human endemic areas: Valley versus altiplanic patterns analysed in liver flukes from sheep from Cajamarca and Mantaro, Peru.

Valero, M.A.; Pérez-Crespo, I.; Khoubbane, M.; Artigas, P.; Panova, M.; Ortiz-Oblitas, P.; Maco, V.; Espinoza, J.R.; Más-Coma, S. (Elsevier Ltd, 2012)

Fascioliasis is a zoonotic parasitic disease caused by Fasciola hepatica and Fasciola gigantica. Of both species, F. hepatica is the only one described in the Americas, mainly transmitted by lymnaeid snail vectors of the ...

Resistance of Fasciola hepatica against Triclabendazole in cattle in Cajamarca (Peru): A clinical trial and an in vivo efficacy test in sheep

Ortiz-Oblitas, P.; Scarcella, S.; Cerna, C.C.; Rosales Loredo, C.; Cabrera, M.; Guzmán, M.; Lamenza, P.; Solana, H. (Elsevier., 2013-07-01)

Fasciolosis caused by Fasciola hepatica, is the most prevalent parasitic disease in dairy cattle from the northern region of Cajamarca, Peru. The control of this parasite is based on the use of Triclabendazole (TCBZ), a ...